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Sequenom spectrochip gen ii chip
Spectrochip Gen Ii Chip, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gen+ii+spectrochip/gen+ii+spectrochip/10__1158_slash_1078___0432__ccr___19___1266-120-25-29
Average 90 stars, based on 1 article reviews
spectrochip gen ii chip - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Purification:

Article Title: Genetic evolution of nevus of Ota reveals clonal heterogeneity acquiring BAP1 and TP53 mutations.
Article Snippet: To take out a personal subscription, please click here More information about Pigment Cell & Melanoma Research at www.pigment.org Genetic evolution of nevus of Ota reveals clonal heterogeneity acquiring BAP1 and TP53 mutations Ana Vivancos, Ginevra Caratú, Judit Matito, Eva Muñoz, Berta Ferrer, Javier Hernández-Losa, Domingo Bodet, Mileidys Pérez-Alea, Javier Cortés, Vicente Garcia-Patos and Juan A. Recio

Article Title: Genomic heterogeneity and efficacy of PI3K pathway inhibitors in patients with gynaecological cancer
Article Snippet: .. After that, each mutation was analyzed as the single-base extension product of a probe that anneals immediately contiguous to the mutation position (primer extension was carried out adding 5.4 pmol of each primer extension probe, 50 Mmol of ddNTP and 0.5 units of Thermosequenase DNA polymerase to the amplification products; reactions were cycled at 94 °C for 2 min, followed by 40 cycles of 94 °C for 5 s, remove residual salt from the reactions, 7 nl of the purified primer extension reaction were loaded onto a matrix pad (3-hydroxypicoloinic acid) of a Gen II SpectroCHIP (Sequenom®). .. Gen II SpectroCHIPs were loaded into a matrixassisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometer (Mass ARRAY, Sequenom®) and spectra were obtained for each of the extension products.

Article Title: Genetic profile of GNAQ -mutated blue melanocytic neoplasms reveals mutations in genes linked to genomic instability and the PI3K pathway
Article Snippet: .. Primer extension was carried out by adding 5.4 pmol of each primer extension probe, 50 mmol of ddNTP and 0.5 units of Thermosequenase DNA polymerase to the amplification products, which were then incubated at 50°C for 5 s and 72°C for 5 s. After the addition of a cation exchange resin to remove residual salt from the reactions, 7 nl of the purified primer extension reaction was loaded onto a matrix pad (3-hydroxypicoloinic acid) of a Gen II SpectroCHIP (Sequenom). .. Gen II SpectroCHIPs were loaded into a matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometer (Mass ARRAY, Sequenom), and spectra were obtained for each of the extension products.

Mass Spectrometry:

Article Title: Genomic heterogeneity and efficacy of PI3K pathway inhibitors in patients with gynaecological cancer
Article Snippet: After that, each mutation was analyzed as the single-base extension product of a probe that anneals immediately contiguous to the mutation position (primer extension was carried out adding 5.4 pmol of each primer extension probe, 50 Mmol of ddNTP and 0.5 units of Thermosequenase DNA polymerase to the amplification products; reactions were cycled at 94 °C for 2 min, followed by 40 cycles of 94 °C for 5 s, remove residual salt from the reactions, 7 nl of the purified primer extension reaction were loaded onto a matrix pad (3-hydroxypicoloinic acid) of a Gen II SpectroCHIP (Sequenom®). .. Gen II SpectroCHIPs were loaded into a matrixassisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometer (Mass ARRAY, Sequenom®) and spectra were obtained for each of the extension products. .. Data analysis and mutation reports were generated using the Typer Analyzer 4.0 software (Sequenom®).

Article Title: Genetic evolution of nevus of Ota reveals clonal heterogeneity acquiring BAP1 and TP53 mutations.
Article Snippet: To take out a personal subscription, please click here More information about Pigment Cell & Melanoma Research at www.pigment.org Genetic evolution of nevus of Ota reveals clonal heterogeneity acquiring BAP1 and TP53 mutations Ana Vivancos, Ginevra Caratú, Judit Matito, Eva Muñoz, Berta Ferrer, Javier Hernández-Losa, Domingo Bodet, Mileidys Pérez-Alea, Javier Cortés, Vicente Garcia-Patos and Juan A. Recio

Article Title: Genetic profile of GNAQ -mutated blue melanocytic neoplasms reveals mutations in genes linked to genomic instability and the PI3K pathway
Article Snippet: Primer extension was carried out by adding 5.4 pmol of each primer extension probe, 50 mmol of ddNTP and 0.5 units of Thermosequenase DNA polymerase to the amplification products, which were then incubated at 50°C for 5 s and 72°C for 5 s. After the addition of a cation exchange resin to remove residual salt from the reactions, 7 nl of the purified primer extension reaction was loaded onto a matrix pad (3-hydroxypicoloinic acid) of a Gen II SpectroCHIP (Sequenom). .. Gen II SpectroCHIPs were loaded into a matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometer (Mass ARRAY, Sequenom), and spectra were obtained for each of the extension products. .. Data analysis and mutation reports were generated using the Typer Analyzer 4.0 software (Sequenom).

Mutagenesis:

Article Title: Genomic heterogeneity and efficacy of PI3K pathway inhibitors in patients with gynaecological cancer
Article Snippet: .. After that, each mutation was analyzed as the single-base extension product of a probe that anneals immediately contiguous to the mutation position (primer extension was carried out adding 5.4 pmol of each primer extension probe, 50 Mmol of ddNTP and 0.5 units of Thermosequenase DNA polymerase to the amplification products; reactions were cycled at 94 °C for 2 min, followed by 40 cycles of 94 °C for 5 s, remove residual salt from the reactions, 7 nl of the purified primer extension reaction were loaded onto a matrix pad (3-hydroxypicoloinic acid) of a Gen II SpectroCHIP (Sequenom®). .. Gen II SpectroCHIPs were loaded into a matrixassisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometer (Mass ARRAY, Sequenom®) and spectra were obtained for each of the extension products.

Amplification:

Article Title: Genomic heterogeneity and efficacy of PI3K pathway inhibitors in patients with gynaecological cancer
Article Snippet: .. After that, each mutation was analyzed as the single-base extension product of a probe that anneals immediately contiguous to the mutation position (primer extension was carried out adding 5.4 pmol of each primer extension probe, 50 Mmol of ddNTP and 0.5 units of Thermosequenase DNA polymerase to the amplification products; reactions were cycled at 94 °C for 2 min, followed by 40 cycles of 94 °C for 5 s, remove residual salt from the reactions, 7 nl of the purified primer extension reaction were loaded onto a matrix pad (3-hydroxypicoloinic acid) of a Gen II SpectroCHIP (Sequenom®). .. Gen II SpectroCHIPs were loaded into a matrixassisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometer (Mass ARRAY, Sequenom®) and spectra were obtained for each of the extension products.

Article Title: Genetic profile of GNAQ -mutated blue melanocytic neoplasms reveals mutations in genes linked to genomic instability and the PI3K pathway
Article Snippet: .. Primer extension was carried out by adding 5.4 pmol of each primer extension probe, 50 mmol of ddNTP and 0.5 units of Thermosequenase DNA polymerase to the amplification products, which were then incubated at 50°C for 5 s and 72°C for 5 s. After the addition of a cation exchange resin to remove residual salt from the reactions, 7 nl of the purified primer extension reaction was loaded onto a matrix pad (3-hydroxypicoloinic acid) of a Gen II SpectroCHIP (Sequenom). .. Gen II SpectroCHIPs were loaded into a matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometer (Mass ARRAY, Sequenom), and spectra were obtained for each of the extension products.

Incubation:

Article Title: Genetic profile of GNAQ -mutated blue melanocytic neoplasms reveals mutations in genes linked to genomic instability and the PI3K pathway
Article Snippet: .. Primer extension was carried out by adding 5.4 pmol of each primer extension probe, 50 mmol of ddNTP and 0.5 units of Thermosequenase DNA polymerase to the amplification products, which were then incubated at 50°C for 5 s and 72°C for 5 s. After the addition of a cation exchange resin to remove residual salt from the reactions, 7 nl of the purified primer extension reaction was loaded onto a matrix pad (3-hydroxypicoloinic acid) of a Gen II SpectroCHIP (Sequenom). .. Gen II SpectroCHIPs were loaded into a matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF) mass spectrometer (Mass ARRAY, Sequenom), and spectra were obtained for each of the extension products.



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Image Search Results


Fig. 4

Journal: Journal of visualized experiments : JoVE

Article Title: Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

doi: 10.3791/63089

Figure Lengend Snippet: Fig. 4

Article Snippet: SpectroCHIP array , Agena Bioscience, CA , #01509 , Protocol 3, 5.

Techniques: Transferring, Mass Spectrometry, Control, Software, Spectrophotometry